Search results for “Ion chromatography

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2 articles

Characterization of Ground Water Quality in Southern and Northern Region of Zanzibar Island

Jun 2021 DOI 10.14302/issn.2377-2549.jndc-21-3740
AJM AbdulCorresponding author Department of Natural Science, School of Natural and Social Sciences, The State University of Zanzibar. P. O. Box 146, Zanzibar-Tanzania.

Millions of people from different countries depend on groundwater, which might contain elevated levels of unhygienic constituents. Poor quality of drinking water in many countries has been forcing people to use bottled water. In the present study, groundwater samples were collected in November 2014 leading to twenty-nine analysis of groundwater samples. The primary objective of this paper is to study the selected groundwater quality parameters as an establishment of preliminary baselines on groundwater constituents. A total of twenty nine water samples were collected (fourteen and fifteen samples from North and South regions of Zanzibar Island respectively. Physical parameters (Turbidity, oxidation-reduction potential ORP, Eh, pH, total dissolved soild TDS, salinity, and Electrical conductivity (EC) were determined in situ using Horiba multiparameters digital meters. Analysis of anions (NO3-, Br- , SO42-, F-, NO2-) was carried out using Ion chromatography (IC). Nitrite and nitrate for some water were below detection limit (BDL) The order of relative abundance for anions in samples collected at Northern region of Zanzibar Island, was NO3- > Br- > SO42- > F- > NO2- , while for the Southern region the order was NO3- >Br- > NO2- > SO42- > F- . In both regions, the orders for other parameters were; Turbidity > pH > salinity; and EC >TDS > ORP > Eh. Almost all the measured parameters were within the WHO guidelines for drinking water. However, for nitrate and bromide, the percentage that exceeded the WHO limit was 7%, and 10.3% respectively. Based on toxicological perspective, although few samples had concentrations above groundwater quality criterion it is fundamental to estimate the intake by humans through the drinking water pathways.

Optimization, Production, Purification of Laccase Enzyme from Bacillus sp

Jul 2020 DOI 10.14302/issn.2377-2549.jndc-20-3460
Tamilvanan NivedharshiniCorresponding author India

Laccase enzyme production is important and more beneficial for environment, because it has many roles like, involved in bioremediation, biodegradation, decolorization of environmental polluted dyes and pharmaceutical sector also. Production of laacse enzyme from bacillus sp as using of Agro waste (rice bran) as a substrate. The Agricultural soil sample was collected, after the sample were processed for the preliminary and biochemical tests to identification of Bacillus organism. The Guiacol inducer were used for microbial screening of laccase enzyme production. After that microbial screening, various optimization parameters (pH, Temperature, Inducers, carbon and nitrogen sources) are checked for that production of laccase enzyme in mass level. Based on that optimization the bulk fermentation (large scale) (solid state fermentation) were done as a rice bran substrate. The fermentation product was subjected to analyzed the physiochemical properties and purification based on that techniques of Gel filtration chromatography, Dialysis, Ammonium sulfate preciptation. The protein estimation of that product to analysed by lowry’s method.

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