New Developments in Chemistry
Open Access
Phytohemagglutinin and Light-Induced Charge Density Effects on Plasma Membrane of PlectonemaBoryanum
Apr 2014 DOI 10.14302/issn.2377-2549.jndc-13-262
Doltchinkova VirjiniaCorresponding author
Department of Biophysics and Radiobiology, Faculty of Biology, 8 Dragan Tzankov Blvd., Sofia University “St. Kliment Ohridski”, 1164 Sofia, Bulgaria
Effects of the plant lectin phytohemagglutinin M (PHA-M) and illumination on the electrophoretic mobility (EPM) and 90°-light scattering of suspension of spheroplasts obtained by lysis of the unicellular diazothrophic cyanobacteriumPlectonemaboryanumwere studied. Cells cultures were grown on media with different iron content: an iron-deficient (denominated as “Fe-starved” culture), an iron-sufficient (“Fe-sufficient”), and a twentyfold iron excess medium (“Fe-excess”). Lectin addition led to a decrease of the membrane charge density and aggregation of the vesicles. Our results indicate significant influence of the Fe2+ and illumination on the lectin-induced decreasing of EPM and light scattering intensity.