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Sep 2018 DOI 10.14302/issn.2641-5526.jmid-18-2328
Fröhlich EckhartCorresponding author
Department of Internal Medicine 1, University of Tübingen, Otfried-Müller-Straße 10, D 72076 Tübingen
Background: Monitoring ultrasonic probe quality remains an important problem which impacts diagnostic accuracy. Here we present a quantitative method to assess probe quality primarily based on measuring probe maximum contrast (dB) and dynamic range. Method: Contrast relevant parameters of 26 transducer models manufactured by five manufacturers were measured with a novel Random Void Phantom (RVP) approach. 3D-data were acquired and analysed to determine image quality. Results: Acoustic contrast values ranging from 15dB to 36dB were observed. Conclusion: By examining artefact producing side lobes, the novel RVP approach presented here permits a quantitative assessment of ultrasound probe quality.
Aug 2018 DOI 10.14302/issn.2640-6403.jtrr-18-2158
Irianov Yu.M.Corresponding author
Russian Ilizarov Scientific Center for Restorative Traumatology and Orthopedic, Russia
Purpose of Study: To study reparative osteogenesis and tissue integration characteristics for implanting three-dimensional mesh structures of titanium nickelide into a bone cavitary defect. Material and Methods: The authors modeled cavitary defects of femoral metaphysis experimentally in Wistar rats divided into an experimental group and control one. The study duration was 60 days in total. The methods of radiography, those of light and electron microscopy, X-ray electron probe microanalysis used. Results: Under implantation the defect was filled with cancellous bone the volumetric density of which more than 1,5-fold exceeded control values (р < 0.001). The implant had biocompatibility, osteoconductive and osteoinductive properties, it stopped inflammatory processes. The membrane protective barrier which prevented connective tissue sprouting was formed on the implant surface in the defect periosteal zone. The osteointegrative junction was formed being persisted up to the end of the experiment. Reparative osteogenesis was performed by direct intramembranous and apposition type. Conclusion: The implant of three-dimensional mesh titanium-nickelide structures has marked osteoplastic properties, and it can be successfully used in orthopedic surgery.
Sep 2024 DOI 10.14302/issn.2575-7881.jdrr-24-5255
O. Henderson JeffreyCorresponding author
This article surveys RBM45 from molecular function and RNA binding to cellular roles and evolutionary conservation. It summarizes emerging links to neurobiology and disease, and highlights tools and models to probe RBM45 biology.
Jul 2024 DOI 10.14302/oap.jc-23-4781
S. Kadandale JayaramaCorresponding author
As a first step towards clarifying the basis of the cooperation and conflict seen in chimeric binary mixes of Dictyostelium giganteum, we examined the karyotype of six natural isolates. All six had 5 haploid chromosomes. No meiotic figures were seen. Fluorescence in-situ hybridization was carried out using conserved D. discoideum centromeric DNA sequences as probes. From it, we infer that two chromosomes are sub-metacentric, one is metacentric and two are telocentric.
Feb 2021 DOI 10.14302/issn.2692-1537.ijcv-20-3644
Lamothe MaraCorresponding author
División de Ciencias de la Salud, Medical Surgeon Career, Universidad Autónoma Metropolitana, Xochimilco, Mexico
We Hypothesize that It should be intuitively inferred that the behavior of the pandemics corresponds to the prediction of the Maxwell Boltzmann distribution, which is a direct function of population density. In a higher organizational metalevel, it is analogous to the Brownian movement effect, in which the possibility of being observed is determined by the observational power of the instrument. In this case, it corresponds to the sensibility and sensitivity of the probe as well as the prevalence of the arbitrary states. The states are formally considered as a partition in probability theory; both in the Kolmogorov and frequentist approach. All these become subsumed to the Bayes theorem applied to recursive conditional probability.
Feb 2021 DOI 10.14302/issn.2692-1537.ijcv-20-3644
Lamothe MaraCorresponding author
División de Ciencias de la Salud, Medical Surgeon Career, Universidad Autónoma Metropolitana, Xochimilco, Mexico
We Hypothesize that It should be intuitively inferred that the behavior of the pandemics corresponds to the prediction of the Maxwell Boltzmann distribution, which is a direct function of population density. In a higher organizational metalevel, it is analogous to the Brownian movement effect, in which the possibility of being observed is determined by the observational power of the instrument. In this case, it corresponds to the sensibility and sensitivity of the probe as well as the prevalence of the arbitrary states. The states are formally considered as a partition in probability theory; both in the Kolmogorov and frequentist approach. All these become subsumed to the Bayes theorem applied to recursive conditional probability.
Nov 2020 DOI 10.14302/issn.2689-2855.jan-20-3616
Hossien Hosseini SeyedCorresponding author
Department of Chemistry, Faculty of Science, Islamshahr Branch, Islamic Azad University, Tehran, Iran, Nika Pooyesh Strategic Sciences Research Institute, NBP Economic Group, Tehran-Iran
The composites of Cu, Carbonyl iron (CI), carbon nanofiber (CNF), graphite nanoflake (GNF)/polypyrrole (PPy) and [(Cu-CI-CNF-GNF) 0.5-PAA]-PPy0.5 were synthesized via different methods by in-situ polymerization on the surface of nanoparticles (NPs) with core-shell structure. This paper describes a method for polyacrylic acid (PAA) coating of NPs in aqueous solution. Then PPy coating was performed by template polymerization on NPs-PAA. Morphology, magnetic and conductivity properties were observed via scanning electron microscopy (SEM), vibrating sample magnetometer (VSM) and four probe method, respectively. The microwave characterization of nanocomposite was evaluated through arch test based on a network analyzer. The PPy nanocomposites possessed the excellent microwave multi absorbers properties in 2-18 GHz. It was also found that nanocomposites with 50% w/w and light weight exhibit good microwave absorbing properties in 2-3 GHz and 5-14 GHz frequency, so can be used to cellphone, radio frequency and radar shielding.
Dec 2019 DOI 10.14302/issn.2577-2279.ijha-19-3110
N.A. KiryanovCorresponding author
Izhevsk State Medical Academy, Izhevsk, Russia
Aim To study the chondroplasty efficacy of the bone matrix obtained using an original technology in restoring cartilage defect of the knee joint. Material and Methods Marginal defects were modeled on the surface of the distal end of the femur in 40 adult male Wistar rats. The bone matrix obtained using an original technology was implanted in the damaged area in animals of the experimental group. Material was investigated by means of light microscopy, transmission and scanning electron microscopy, and electron probe X-ray microanalysis. Results It was found that the bone matrix implanted did not cause an immune rejection reaction, activated reparative chondrogenesis for a prolonged period. In the area of articular cartilage lesion, the regenerate acquiring cellular and histochemical characteristics of the hyaline cartilage tissue was formed. The chondroinductive properties for the bone matrix were ensured by localized growth factors and morphogenetic proteins released during osteoclastic resorption. Conclusion The application of the bone matrix as a stimulator of chondrogenesis is theoretically reasonable and has a good perspective in treatment of damages and diseases of the articular cartilage.
May 2019 DOI 10.14302/issn.2639-3166.jar-19-2780
Masoero GiorgioCorresponding author
Accademia di Agricoltura di Torino, Italy
The inoculation of soil with a bio-fertilizer (BF), with arbuscular mycorrhiza fungi, characterizes a Symbiotic (S) agriculture mode, aimed at promoting the yield and health of crops through modifications in the rhizosphere as well as in the plant phenotype. The main objective of this study was to reduce the incidence of Olive Quick Decline Syndrome (OQDS, involving Xylella fastidiosasubsp.pauca) that afflicts the olive groves in Apulia (Italy). Non-inoculated control (C) plants were compared with Symbiotic (S) plants inoculated with 20 kg ha-1 of Micosat F ®, through a 15 cm deep scarification, in the groves of seven farms covering an area of 27 ha. In addition to a visual observation of 484 plants, to obtain a gradation of the disease severity, some objective rapid type methods were utilized to survey the plants and soil , namely leaf pH, NIR tomoscopy of the leaves, hay-litter-bag probes coupled with NIR spectroscopy and the prediction of soil induced respiration. The fingerprinting of the S and C types of leaves and litter-bags was ascertained by means of the use of a random forest algorithm in the classification matrices. The results on the symptoms appeared variable: they were significantly mitigated in two groves out of six, but they were aggravated in one. All the rapid measurements became essentials in a “holistic” model which was able to explain over 95% of the average mitigation / null / aggravation response to BF inoculation. The holistic model gathers differential and compositional analyses of the leaf (pH, crude protein, water) and of the soil (respiration), but depends mainly on the fingerprinting of the C and S leaves and litter-bags. Two keys were identified for a successful inoculation: a high degree of variability of the soil conditions permitting hospitality for the BF with enhancement of the microbial activity in the S soil (lowering the fingerprint of the control litter-bags) and homogeneity of the leaves (with increases in the fingerprint of the S leaves treated with BF). In short, the inoculation of diseased plants with one BF consortium is far from being the ultimate remedy to mitigate OQDS in all situations. Further studies are needed, at a field level, to clarify the soil hosting capacity and to define the mycorrhizal and / or endophytic * plant * pathogen interactions, even using rapid methods.
Mar 2019 DOI 10.14302/issn.2690-4721.ijcm-19-2616
Srinath Kamineni,Corresponding author
Department of Orthopaedics and Sports Medicine, Elbow Shoulder Research Center, University of Kentucky, Lexington, KY 40536
Introduction Ultrasound technology has previously been applied for cataract removal and tennis elbow treatment. Recent data supports the use of ultrasonic debridement in the treatment of diabetic foot ulcers. No data is available concerning the potential antibacterial properties of a clinical grade, lower energy ultrasound probe. We investigated the effect of a ultrasonic probe with respect to P gingivalis bacterial viability. Methods A Tenex Tx1 probe with standard settings for clinical use was used for this study. A Gram negative (Porphyromonasgingivalis) bacteria, known for its pathological activity, was investigated. The bacteria was cultured in an anaerobic broth, re-suspended to achieve a consistent bacterial count, and 5ml of this re-suspension was placed in a test tube for testing. Each tube was sonicated with the Tx1 probe for varying lengths of time (10, 30, 60, 120 seconds). The sonicated was diluted and plated on blood-agar plates, followed by incubation for 48 hours at 37°C in an anaerobic growth chamber. The number of colony forming units were counted, on each plate and the anti-bacterial effect was calculated. A one way analysis of variance was performed for statistical analysis. Results A significant time-dependent antibacterial effect was demonstrated with sonication. When comparing the kill rate between the control and 120 seconds of sonication P Gingivalis had a 64% kill rate. This was the only statistically significant time comparison achieved, although the trend for all the time intervals was a reduction in the colony forming unit counts. Conclusion This study demonstrates that a clinically available ultrasonic probe (Tenex Tx1) has an antibacterial effect against the gram negative anaerobic bacterial species P gingivalis. Complete deactivation was not achieved, and there was a variation in effect dependent on the time of active sonication, with greater sonication times leading to greater kill rates. This data may partially help to explain the ability for ultrasonic debridement to result in the healing of long standing diabetic ulcers, that have been recalcitrant to other forms of treatment.
May 2018 DOI 10.14302/issn.2639-3166.jar-18-2084
Masoero GiorgioCorresponding author
Accademia di Agricoltura di Torino, Torino, Italy
The biofertilization of cropsusing microbial biota in the soil (MBS) is a modern practice that is used to sustain fertility. MBS agents can promote the yield and health of crops, by luxuriating in the shoot as well as in the root systems. Farmers devoted to systematic MBS fertilization are creating a “Symbiotic” (S) form of agriculture, which offers a greater advantage of resilience than Conventional (C) or organic farming. Since MBS is involved in organic matter degradation, hay-litter-bag probes can be used to reflect a global functionality of the active soil, in the short-medium term. It is here shown that the NIRS hay-litter-bag technique, intended not as mass decay but as a quality evolution of the hay probes, can be modelled as a valid footprint of S vs. C soils. A patented MBS was used in eight experiments in which litter-bags from an S treated thesis were compared with equivalent litter-bags from a non-inoculated C thesis. The chemical signature of the S vs. C in the litter-bag composition was a percentage decrease of sugars and fibres. A smart NIRS device was used to discriminate the origin of the S vs. C litter-bags and a sensitivity of 71% (P<0.0001) was obtained. External validations on 37 S farms showed that three NIRS models discriminated the true positive S spectra, with a sensitivity of 90% as single and 98% as compound probabilities The NIRS radiation of the hay-litter-bags confirmed the results of the S vs. C agriculture soil footprint. Moreover, the SCIO-NIR devices also made it possible to connect the S farms in a smart network.
Apr 2018 DOI 10.14302/issn.2577-2279.ijha-18-2030
A. Kiryanov N.Corresponding author
Izhevsk State Medical Academy, Russia.
Introduction: The use of non-medicinal facilities for correcting processes in various pathological conditions is one of the most urgent problems of modern medicine. Purpose of the Work: To study the effect of low-intensity infrared laser radiation on reparative bone formation and angiogenesis in bone regeneration which is formed in treatment of fractures under conditions of transosseous osteosynthesis. Material and Methods: A tibia fracture was modeled experimentally in rats in the control and experimental groups. Reposition and fixation of fragments were performed. The fracture zone in the experimental group animals was exposed to the impact of pulsed infrared laser irradiation of low intensity. Animals from the control group underwent the impact simulation. The operated bones were investigated using the methods of X-ray, light and electron microscopy, X-ray electron probe microanalysis. Results: It was established that laser radiation exposure sessions activated fibrillogenesis and angiogenesis, accelerated compacting of newly formed bone tissue and increased its maturity while primary fracture healing occurred. Prolonged capillary dilatation and endothelium-dependent vasodilation, intensive capillarogenesis were noted after sessions of laser therapy in bone regeneration. Endothelial outgrowth was formed in the lumen of the vessels forming capillary buds that propagate along the “mother” vessels (endovascular capillarogenesis). Conclusion: The data obtained revealed a possible mechanism of laser radiation exposure at the level of a whole organism and proved the effectiveness of its application in clinical practice at the early stages of patient rehabilitation under conditions of transosseous osteosynthesis.
Apr 2018 DOI 10.14302/issn.2578-8590.ipj-18-2078
Kakitsuba NaoshiCorresponding author
Department of Environment and Technology, School of Science and Technology, Meijo University, Nagoya, Japan
Background: The core interthreshold zone (CIZ) is defined as the range between core temperature (Tc) at the onset of shivering and the Tc at the onset of sweating under consistent mean skin temperatures of 28°C to 30°C. A previous study demonstrated a diurnal change in the CIZ for male subjects and its relationship to the cutaneous sensation threshold zone (CSZ). In the present study, diurnal changes in the CIZ and the CSZ for young Japanese female subjects were investigated using the same experimental protocol from the study of male subjects and the sex differences in these responses were then examined. Methods: The CIZ and the CSZ were measured in 10 female subjects who participated in three experiments in a single day during the morning, afternoon, and evening in the summer of 2014 (single-day experiment), and six female subjects who participated in the same experiments on the morning of day 1, the afternoon of day 2, and the evening of day 3 during the summer of 2016 (multiple-day experiment). Air temperature was controlled at 25°C. Each subject wore a suit perfused with 25°C water at a rate of 600 cc/min, and exercised at 50% of their maximum work rate on an ergometer for 10–15 min until their sweating rate increased. They then remained seated, without exercising, until their oxygen uptake increased. Rectal temperature, skin temperature at seven sites, the forehead-sweating rate, and oxygen uptake were continuously monitored throughout the experiment. Cutaneous warm and cold sensation thresholds were measured at three sites using 1-cm2 and 2-cm2 probes. Results: The results from the single-day experiment demonstrated that the CIZ was proportional to core temperature prior to exercise (Tc-init) whereas the results from the multiple-day experiment demonstrated that the CIZ increased continuously from morning to evening despite almost a constant Tc-init. The CIZ appeared to be proportional to the CSZ measured with the 2-cm2 probe. When compared with the results from the previous study of men, no significant sex difference was observed between the CIZ of 0.25±0.07°C for female subjects and 0.21±0.05°C for male subjects. Conclusion: No significant sex difference or diurnal variation in the CIZ was confirmed. Continuous increase in the CIZ from morning until evening is expected in both men and women under a normal Tc circadian rhythm.
Feb 2018 DOI 10.14302/issn.2577-2279.ijha-18-1918
Kiryanov N.A.Corresponding author
Izhevsk State Medical Academy, Russia
Purpose of Study To study reparative osteogenesis and tissue integration characteristics for implanting three-dimensional mesh structures of titanium nickelide into a bone cavitary defect. Material and Methods The authors modeled cavitary defects of femoral metaphysis experimentally in Wistar rats divided into an experimental group and control one. The study duration was 60 days in total. The methods of radiography, those of light and electron microscopy, X-ray electron probe microanalysis used. Results Under implantation the defect was filled with cancellous bone the volumetric density of which more than 1,5-fold exceeded control values (р < 0.001). The implant had biocompatibility, osteoconductive and osteoinductive properties, it stopped inflammatory processes. The membrane protective barrier which prevented connective tissue sprouting was formed on the implant surface in the defect periosteal zone. The osteointegrative junction was formed being persisted up to the end of the experiment. Reparative osteogenesis was performed by direct intramembranous and apposition type. Conclusion The implant of three-dimensional mesh titaium-nickelide structures has marked osteoplastic properties, and it can be successfully used in orthopedic surgery.